How to describe a cyto slide?

Objective 10x:

Scanning the whole slide for general information!

=> Do I have a diagnostic slide?

·      Background: colour (protein content), presence of blood, debris (nuclear streaming, …), other material (fat,…)

·      Stain property (eosinophil & basophil

·      Searching cell material:

o   spread of cells => Can I find monolayer areas?

o   cellularity and cell preservation => Do I find enough, intact cells?

·      Looking for bigger parts (Platelet aggregats, microfilaria larvae in a skin nodule, extracellular pigment, ect.)

 

Objective 40x

Get the main information about cell type and dignity!

o   How many cell types are on the slide?

o   What is the main cell population?

§  Neutrophils, Monocytes, Lymphocytes, Eosinophiles

=> inflammation

§  Other cells:

·      cell type and shape: round, epithelial, spindle, naked nuclei, undifferentiated => tumor

§  Cell arrangements:

·      single cells or groups, palisade, acinar, row, ect.) and cohesiveness

§  Cell borders:

·      well defined, indistinct ect.

o   Criteria of malignancy (COM):

§  General and Nuclear: Anisocytosis, Anisokaryosis, Macrocytosis, Macrokaryosis, ect.

 

Objective 100x oil

Looking for more small details and nuclear/nucleolar COM!

       cytoplasm

       Nuclei, chromatin pattern

       Nucleoli

       Inclusion bodies

       Infectious agents......

 

Always keep in mind:

-       location of sampling => What tissue can I expect?

-       never try to interpret naked nuclei => Looking for intact cells with cytoplasma!

 

 

GOOD LUCK!

Kontakt

DVM Stefanie Valentina Knöpfler, Msc

FTÄ für Klinische Laboratoriumsdiagnostik (ESVCP)

FTÄ für Dermatologie der Klein- und Heimtiere (ESVD)

 

 

email: vet.zyto.hd@gmail.com

mobil: 0049 176 257 155 08

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